Denaturation pūmua

Conformation (hanganga) o te mekameka peptide whakahau te me he ahurei ki ia pūmua. I roto i ngā āhuatanga motuhake i reira he he maha nui āputa o here e ū ai te hanganga mokowā o te rāpoi ngota pūhui. Ka rite ki te hua, te āputa tino te muingota katoa (ranei te wahi nui ona) e te ahua o te pōkai tupurangi. huaina ana tēnei tukanga ko "denaturation". Ka taea e tēnei huringa tīmata te wera i ono tekau ki te waru tekau nga nekehanga. Ko te kupu, ia rāpoi ngota, kia rerekē te āputa hua i roto i conformation i te tahi atu.

Denaturation o pūmua e wahi i raro i te mana o tetahi māngai taea o whakangaro kore-kotuari here. Tenei tukanga kia puta i raro i ngā āhuatanga kawakore waikawa ranei, i runga i te mata o te wāhanga wāhanga, i raro i te mahi o te tahi mau pūhui matū waro (phenols, waiwaihā me ētahi atu). Ka taea e te pūmua denaturation puta i raro i te mana o te guanidinium pūhaumāota Urea ranei. āpiha mea hanga i here ngoikore (hydrophobic, ngota, hauwai) ki carbonyl amino ranei ngā rōpū o te tapahanga peptide ki te maha o ngā rōpū o ngā toenga waikawa amino, hei whakakapi ratou ake wātea pūmua here hauwai i roto i te rāpoi ngota. Ka rite ki te hua, puta he huringa i roto i te hanganga tuarua, me te tuatoru.

Ātete ki āpiha denaturing tei te nuinga i runga i te aroaro i roto i te rāpoi ngota o te pūhui pūmua o here disulfide. He Trypsin inhibitor toru here S - S. I raro i te huru o te ora o te pūmua denaturation puta kahore atu awe. Ki te hoatu muri te hononga i roto i ngā āhuatanga i reira kawea te hāora i roto i SH-rōpū o cysteine me hanganga o here disulfide, whakahokia te conformation taketake te. Ko te aroaro o ara te here disulfide kotahi tino whakanuia ana te pūmautanga o te hanganga mokowā.

pūmua denaturation, nuinga haere tahi e te hekenga i roto i te memehatanga. Fakataha ki tenei te hohenga ka maha puka. Puta te reira i roto i te puka o "pūmua tetepe." I ngä nohanga tiketike o te pūhui i te otinga, "coagulation" te whakamamaetia te otinga katoa, rite, mo te tauira, e tupu tenei ina kohua hua. A, no te ngaro denaturation o te pūmua tona mahi koiora. hāngai ana tēnei parau tumu e te whakamahi o te waikawa carbolic (phenol wairewa) rite te taonga rongoā horoi.

Ko te tapepe o te hanganga mokowā, he tino uaua ki te tutakina me te rangahau pūmua te tūponotanga teitei o te kore i raro i te mana o ngā māngai. hanga ano e etahi raruraru, no te whakamahi i te pūhui i roto i te rongoa me te ahumahi.

Ki te kawea te denaturation pūmua i roto i te te rongo ki pāmahana teitei, e tautauā puhoi wahi i roto i etahi tikanga te renativatsii tukanga - whakaora o te whenua (taketake) conformation. Tenei meka te akapapu e te whakapanga o te mekameka peptide kei roto i runga i te hanganga tuatahi.

Ko te hanganga o te conformation taketake (wāhi pūtake) ko he tukanga māhorahora. I roto i te mau parau te tahi atu, hāngai tenei whakaritenga ki te nui iti o te pūngao noa i roto i roto i te rāpoi ngota. Ka taea e rite ki te hua kia faaoti tetahi e whakawaeheretia te hanganga mokowā o te pūhui e i roto i te raupapa waikawa amino i roto i nga mekameka peptide. i roto i te tahuri te tikanga tenei e polypeptides katoa e pā ana i te whakarerekētanga o waikawa amino (hei tauira, mekameka mioglobinovye peptide), ka tango i te conformation ōrite.

Ka taea e pūmua e nui ngā rerekētanga i roto i te hanganga tuatahi, ara, ki te iti ranei tino ōrite i roto i te conformation.